Effect of microbial consortium on plant growth and improvement of alkaloid content in Withania somnifera (Ashwagandha)

The effect of microbial consortium consisting Plant Growth Promoting Rhizobacteria (PGPR) like Azospirillum, Azotobacter, Pseudomonas and Bacillus were tested separately and in combination on Withania somnifera for two consecutive years (2009 and 2010). The combinations of above mentioned PGPR strains significantly increased plant height, root length, and alkaloid content in Withania somnifera when compared to the uninoculated control. Plant growth promoting rhizobacteria (PGPR) exhibit direct and indirect mechanisms as plant growth promoters and biological control agents. Direct mechanism by PGPR, include the provision of bio-available phosphorus for plant uptake, nitrogen fixation for plant. The results of this study suggest that the PGPR applied in combination have the potential to increase the plant growth, alkaloid content of Withania somnifera

Estimation of alkaloid content of Ashwagandha (Withania somnifera) with HPLC methods

Withania somnifera (Family; Solanaceae) is commonly known as Ashwagandha and is used in traditionally system of medicine since long time. Many compounds have been reported from the root part of the plant. In present study, comparative evaluation of microbial consortium and uninoculated control plant root collected from different geographically sources was done by HPLC. Methanol soluble root extract of the plant was subjected to Column chromatography to isolate and purify the phyto-constituents which can be termed as markers. Three compounds were isolated and purified. Compounds were characterized as withanone and withaferin-A. The third compound was found to be impure. Novel HPLC methods were developed for assessment of purity, for standardization and for estimation of the compounds. Simplicity of isolation and HPLC analysis for the compounds suggests that the compounds may be termed as markers for the standardization of the methanol extracts and preparations containing Withania somnifera

Population dynamics of exotic rugose spiralling whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) on coconut as influenced by weather factors and natural enemies

The rugose spiralling whitefly, Aleurodicus rugioperculatus Martin is a new exotic pest occurring in several crops including coconut since 2016 in India. Due to variation in the agro-climatic conditions of different regions, arthropods show varying trends in their incidence also in nature and extent of damage to the crop. Besides, abiotic factors also play a key role in determining the incidence and dominance of a particular pest and their natural enemies in a crop ecosystem. The population dynamics of new exotic whitefly species, A. rugioperculatus and their associated natural enemies was assessed on five-year-old Chowghat Orange Dwarf coconut trees at Coconut Farm of Tamil Nadu Agricultural University. The study indicated that RSW was found throughout the year on coconut and the observation recorded on weekly interval basis shows that A. rugioperculatus population escalated from the first week of July 2018 (130.8 nymphs/leaf/frond) reaching the maximum during the first week of October (161.0 nymphs/leaf/frond) which subsequently dwindled to a minimum during April. The parasitisation by E. guadeloupae on RSW ranged from 31.60 percent in Aug. 2018 to 57.60 percent in December 2018. The association of biotic and abiotic factors with A. rugioperculatus population showed a negative correlation with E. guadeloupae and C. montrouzieri. There was a significant positive correlation between maximum temperature and minimum temperature as well as relative humidity. However, rainfall showed a negative correlation with A. rugioperculatus population

On Characterizing the Data Access Complexity of Programs

Technology trends will cause data movement to account for the majority of energy expenditure and execution time on emerging computers. Therefore, computational complexity will no longer be a sufficient metric for comparing algorithms, and a fundamental characterization of data access complexity will be increasingly important. The problem of developing lower bounds for data access complexity has been modeled using the formalism of Hong & Kung's red/blue pebble game for computational directed acyclic graphs (CDAGs). However, previously developed approaches to lower bounds analysis for the red/blue pebble game are very limited in effectiveness when applied to CDAGs of real programs, with computations comprised of multiple sub-computations with differing DAG structure. We address this problem by developing an approach for effectively composing lower bounds based on graph decomposition. We also develop a static analysis algorithm to derive the asymptotic data-access lower bounds of programs, as a function of the problem size and cache size

Synthesis and Characterization of Novel Schiff base Cu(II) Complexes: Antimicrobial and Molecular docking Studies

N2O2 type complexes of C+2uion have been synthesized by the reaction of Salicylaldehyde/ 3,4-diaminobenzophenone/ acetyl acetoneand glutaric anhydride. The ligands and respective metal complexes was established through spectroscopic data (FT-IR, UV-Vis,1H NMR and 13C NMR). They are non-electrolytic in nature as their molar conductivities (ΛM) in DMSO of 10-3 M solution from the EPR study the complexes proposed to be octahedral geometry. All the metal complexes have been screened for their antibacterial activity andthe predicted binding affinity using molecular docking studies

Geochemical and isotopic signatures for the identification of seawater intrusion in an alluvial aquifer

Seawater intrusion is one of the alarming processes that reduces the water quality and imperils the supply of freshwater in coastal aquifers. The region, north of the Chennai city, India is one such site affected by seawater intrusion. The objective of this study is to identify the extent of seawater intruded area by major geochemical and isotopic signatures. A total of 102 groundwater samples were collected and analysed for major and minor ions. Groundwater samples with electrical conductivity (EC) greater than 5000 μS/cm and a river mouth sample were analyzed for Oxygen-18 (δ 18O) and Deuterium (δ 2H) isotopes to study their importance in monitoring seawater intrusion. The molar ratio of geochemical indicators and isotopic signatures suggests an intrusion up to a distance of 13 km from the sea as on March 2012 and up to 14.7 km during May 2012

Practices towards prevention of food borne diseases among females in rural area of Trichy, India

Background: Food borne diseases are of great importance since it leads to multiple health problems resulting in illness, malnutrition, mortality and economic loss. These diseases share a significant proportion in the occurrence of communicable diseases. Objectives was to study the knowledge about food borne diseases and food safety among women residing in rural area.Methods: This was a cross sectional study involving 150 adult females who were residing in rural field practice areas of a tertiary care teaching hospital. Practices of safe food handling were assessed using an interviewer administered questionnaire. Informed consent was obtained, and confidentiality was maintained. Appropriate descriptive statistical analysis was done.Results: Out of 150 females, 147 (98%) were aware of the importance of washing hands before cooking. Sixty four (42.7%) females had a habit of washing the vegetables with water twice before cooking. 102(68%) females consumed stored food after reheating, 48(32%)females consumed without heating. Though 70% were aware of food adulteration, none of the participants have lodged compliant against adulteration.Conclusions: Through our study we found that most of the females practiced proper hand washing before cooking and proper storage of the leftover food. The proportion of females who followed adequate cleaning of non-vegetarian food items and vegetables were found to be inadequate.

Subsurface Hydrogeochemical Processes in Lower Bhavani River Basin, Tamil Nadu, India

Bhavani River is one of the important tributaries of Cauvery River, and originates in the Silent Valley range of Kerala State, India. The Lower Bhavani River Basin lies between 11 15' N and 11 45' N latitudes and 77 00' E and 77 40' E longitudes with an area of 2,475 km2. Variation of groundwater quality in an area is a function of physical and chemical parameters that are greatly influenced by geological formations, recharge-discharge mechanisms of groundwater and anthropogenic activities. The correlation of groundwater chemistry with hydrologic and geologic environments gives valuable information to understand the effect of these processes and to properly manage aquifer systems. A detailed study has been carried out to understand the subsurface hydrogeochemical processes that are responsible for the quality variation of groundwater. Residence time of groundwater was also considered to be an important parameter to study groundwater evolution. The NETPATH computer code was used to model the major subsurface processes contributing to the evolution of groundwater chemistry. The occurrence of such chemical processes as silicate weathering, carbonate dissolution, ion exchange and dilution due to rain were verified by performing inverse mass balance modeling using the same code. The net geochemical mass balance reactions between initial and final water were identified and quantified based on the flow in selected well pairs. The model output shows that dilution, ion exchange and illite precipitation are the dominant processes that control the chemistry of the groundwater along the flow paths. Calcite and NaCl dissolution are also involved to a certain extent. Reverse ion exchange process is also observed in two models